RRPL Compendium of Tests

Expanded Respiratory Viral Panel NAAT

Section:

Molecular Diagnostics

Test:

Expanded Respiratory Viral Panel NAAT

Specimen	Collection Procedure
Nasopharyngeal Swab	Nasopharyngeal Swab: Insert the Copan flocked swab into the nasal passage until a slight resistance is met. Rotate the swab two or three times to collect ample epithelial cells. Hold the swab in place for 5 seconds to ensure maximum absorbency. Carefully remove the swab, place it in the Universal Transport Media (UTM) and break the shaft off at the breakpoint.
Throat Swab	Throat Swab: Use a tongue depressor to depress tongue to prevent contamination of swab with saliva. Using a sterile Copan flocked swab vigorously rub the tonsillar area and posterior nasopharnyx. Place swab in Universal Transport Media (UTM).

Specimen

Collection Procedure

Nasopharyngeal Swab

Nasopharyngeal Swab:  Insert the Copan flocked swab into the nasal passage until a slight resistance is met.  Rotate the swab two or three times to collect ample epithelial cells.  Hold the swab in place for 5 seconds to ensure maximum absorbency.  Carefully remove the swab, place it in the Universal Transport Media (UTM) and break the shaft off at the breakpoint.

Throat Swab

Throat Swab:  Use a tongue depressor to depress tongue to prevent contamination of swab with saliva.  Using a sterile Copan flocked swab vigorously rub the tonsillar area and posterior nasopharnyx.  Place swab in Universal Transport Media (UTM).

Requisition:

Microbiology Doc 1400-55 RRPL 001R V11

Special Handling Instructions:

NOTE: The Expanded Respiratory Viral Panel (ERVP) tests for the following viruses: SARS-CoV-2, Influenza A, Influenza B, Respiratory Syncytial Virus (RSV), Adenovirus (AdV), Metapneumovirus (MPV), Rhinovirus (RV), Parainfluenzaviruses 1-4 (PIV-1 to PIV-4), and seasonal coronaviruses (HCoVs)229E, HKU1, NL63, OC43.

NOTE:  The ERVP is not offered routinely for outpatients.  If testing is indicated, call the Microbiologist on-call for approval.

NOTE: If Avian influenza (H5N1, H7N9, etc) or MERS coronavirus are suspected, please call the Microbiologist on-call to arrange appropriate testing as this panel will not distinguish Avian influenza from seasonal, and it will not detect MERS-CoV.

NOTE: The ERVP does NOT include:  Bordetella pertussis or Enterovirus (EV).
If testing is needed for Whooping cough (Bordetella pertussis):
∙ For PCR testing on nasopharyngeal swabs, refer to 'Pertussis NAAT'
∙ For culture and antibiotic sensitivity, refer to 'Bordetella Culture'

NOTE: Send information including outbreak number, location and type of outbreak to RRPL Outbreaks
 prior to submitting specimens from a suspected outbreak.  No more than 6 specimens should be submitted for ERVP testing on outbreaks.

Shipping:

Ship specimens at 4°C. Package as required by transport system used. Ship in accordance with Transport Canada Regulations.

Reference Values:

Positive or Negative

NOTE: Positive SARS-CoV-2, Influenza A, and Influenza B results are reported to a Medical Health Officer.

Turn Around Time:

1 day (after receipt in lab)

Last Modified Date:

10/3/2022 12:05:37 PM

Paper copies of the RRPL Test Compendium are uncontrolled and may be incomplete or out-of-date. To view the most current information, visit https://rrpl-testviewer.ehealthsask.ca/.